Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Reduced immunoproteasome formation and accumulation of immunoproteasomal precursors in the brains of lymphocytic choriomeningitis virus-infected mice.

doi: 10.4049/jimmunol.1001517

Figure Lengend Snippet: FIGURE 6. Colocalization of LMP2 and MECL-1 with the microglia marker Iba-1 in the brain of LCMV-infected BALB/c mice. Coronal brain cry- osections of LCMV-infected BALB/c mice and MECL-12/2/LMP22/2 double knockout mice were costained for LMP2 or MECL-1, which was visualized using a cy3-coupled anti-rabbit Ab (red), and the microglial cell marker Iba-1, which was visualized using an Alexa 488-coupled anti-goat Ab (green). A, Costaining of LMP2 and Iba-1. B, Costaining of MECL-1 and Iba-1. The magnifications of shown images range from 3100 to 3400. The experiments were performed four times yielding similar images.

Article Snippet: Visualization was performed using cy3-conjugated donkey anti-rabbit IgG (1:1000; product No. 711-166-152; Jackson ImmunoResearch, Suffolk, U.K.), cy3-conjugated donkey anti-rat IgG (1:1000; product No. 712-166-150; Jackson ImmunoResearch), Alexa 488-conjugated donkey anti-goat IgG (1:1000; product No. A11055; Invitrogen, Karsruhe, Germany), and Alexa 488-conjugated goat anti-mouse IgG (1:1000; product No. A11017; Invitrogen).

Techniques: Marker, Infection, Double Knockout